Project
SgsE|LigA

Part:BBa_K525005

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-13)

Fusion Protein of S-Layer SgsE and LigA

Fusion protein of SgsE || LigA (RFC 25) for S-layer immobilized, cell-free NAD+-detection after expression in E. coli using T7 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
    Illegal BglII site found at 1022
    Illegal BglII site found at 3819
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 4417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1657
    Illegal SapI.rc site found at 4033


[edit]
Categories
//cds/enzyme/ligase
//chassis/prokaryote/ecoli
//proteindomain/internal
Parameters
biologyS-Layer
chassisE. coli
controlPT7
originGeobacillus stearothermophilus, Escherichia coli