Project
SgsE|LigA
Part:BBa_K525005
Designed by: Anna Drong Group: iGEM11_Bielefeld-Germany (2011-09-13)
Fusion Protein of S-Layer SgsE and LigA
Fusion protein of SgsE || LigA (RFC 25) for S-layer immobilized, cell-free NAD+-detection after expression in E. coli using T7 promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 167
Illegal BglII site found at 1022
Illegal BglII site found at 3819 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 76
Illegal AgeI site found at 4417 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1657
Illegal SapI.rc site found at 4033
[edit]
Categories
Parameters
//cds/enzyme/ligase
//chassis/prokaryote/ecoli
//proteindomain/internal
//chassis/prokaryote/ecoli
//proteindomain/internal
biology | S-Layer |
chassis | E. coli |
control | PT7 |
origin | Geobacillus stearothermophilus, Escherichia coli |